general tubulin buffer Search Results


tubulin buffer  (Cytoskeleton Inc)
95
Cytoskeleton Inc tubulin buffer
A Illustration of experimental design: the 3-week-old mice were injected i.p. with 12 mg/Kg (body weight) paclitaxel (PTX). After 4 hours, the mice were exposed to ultrasound (1 W/cm 2 , 45 kHz) for 5 min, and euthanized, biopsied, and assayed 10 min later. The experiments were repeated 3 times with similar results, and 6 mice in each group ( n = 6) were used. B Skin biopsies were analyzed by histology technique, and the hair follicle sections stained with <t>alpha-Tubulin</t> were shown for examples of cross-section (upper panel) and sagittal section (lower panel). C Areas of the hair follicles were shown in high magnification to visualize microtubules stained with anti-alpha-tubulin. Images were processed using NeuronJ from ImageJ, employing vectorization and skeletonization techniques to measure microtubule length. The lower panel shows images of microtubule vectorization. D The length of microtubule filaments was measured using an image analytical program. “*” p < 0.01; “****” p < 0.001. E Examples were shown for mitotic figures, and shown in higher magnification in the lower panel. F Quantitation of normal, abnormal, and disrupted mitotic figures in cells of the hair follicles in the skin biopsies.
Tubulin Buffer, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+tubulin+buffer/General+tubulin+buffer/pmc13473585-167-20-22
Average 95 stars, based on 1 article reviews
tubulin buffer - by Bioz Stars, 2026-10
95/100 stars
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bovine brain tubulin  (Cytoskeleton Inc)
95
Cytoskeleton Inc bovine brain tubulin
A Illustration of experimental design: the 3-week-old mice were injected i.p. with 12 mg/Kg (body weight) paclitaxel (PTX). After 4 hours, the mice were exposed to ultrasound (1 W/cm 2 , 45 kHz) for 5 min, and euthanized, biopsied, and assayed 10 min later. The experiments were repeated 3 times with similar results, and 6 mice in each group ( n = 6) were used. B Skin biopsies were analyzed by histology technique, and the hair follicle sections stained with <t>alpha-Tubulin</t> were shown for examples of cross-section (upper panel) and sagittal section (lower panel). C Areas of the hair follicles were shown in high magnification to visualize microtubules stained with anti-alpha-tubulin. Images were processed using NeuronJ from ImageJ, employing vectorization and skeletonization techniques to measure microtubule length. The lower panel shows images of microtubule vectorization. D The length of microtubule filaments was measured using an image analytical program. “*” p < 0.01; “****” p < 0.001. E Examples were shown for mitotic figures, and shown in higher magnification in the lower panel. F Quantitation of normal, abnormal, and disrupted mitotic figures in cells of the hair follicles in the skin biopsies.
Bovine Brain Tubulin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+tubulin+buffer/General+Tubulin+Buffer/us09334242-2275-3-8
Average 95 stars, based on 1 article reviews
bovine brain tubulin - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

Image Search Results


A Illustration of experimental design: the 3-week-old mice were injected i.p. with 12 mg/Kg (body weight) paclitaxel (PTX). After 4 hours, the mice were exposed to ultrasound (1 W/cm 2 , 45 kHz) for 5 min, and euthanized, biopsied, and assayed 10 min later. The experiments were repeated 3 times with similar results, and 6 mice in each group ( n = 6) were used. B Skin biopsies were analyzed by histology technique, and the hair follicle sections stained with alpha-Tubulin were shown for examples of cross-section (upper panel) and sagittal section (lower panel). C Areas of the hair follicles were shown in high magnification to visualize microtubules stained with anti-alpha-tubulin. Images were processed using NeuronJ from ImageJ, employing vectorization and skeletonization techniques to measure microtubule length. The lower panel shows images of microtubule vectorization. D The length of microtubule filaments was measured using an image analytical program. “*” p < 0.01; “****” p < 0.001. E Examples were shown for mitotic figures, and shown in higher magnification in the lower panel. F Quantitation of normal, abnormal, and disrupted mitotic figures in cells of the hair follicles in the skin biopsies.

Journal: Nature Communications

Article Title: Disruption of microtubules with low intensity ultrasound rescues hair follicle damage by paclitaxel in mouse models

doi: 10.1038/s41467-026-75335-8

Figure Lengend Snippet: A Illustration of experimental design: the 3-week-old mice were injected i.p. with 12 mg/Kg (body weight) paclitaxel (PTX). After 4 hours, the mice were exposed to ultrasound (1 W/cm 2 , 45 kHz) for 5 min, and euthanized, biopsied, and assayed 10 min later. The experiments were repeated 3 times with similar results, and 6 mice in each group ( n = 6) were used. B Skin biopsies were analyzed by histology technique, and the hair follicle sections stained with alpha-Tubulin were shown for examples of cross-section (upper panel) and sagittal section (lower panel). C Areas of the hair follicles were shown in high magnification to visualize microtubules stained with anti-alpha-tubulin. Images were processed using NeuronJ from ImageJ, employing vectorization and skeletonization techniques to measure microtubule length. The lower panel shows images of microtubule vectorization. D The length of microtubule filaments was measured using an image analytical program. “*” p < 0.01; “****” p < 0.001. E Examples were shown for mitotic figures, and shown in higher magnification in the lower panel. F Quantitation of normal, abnormal, and disrupted mitotic figures in cells of the hair follicles in the skin biopsies.

Article Snippet: Briefly, one aliquot of 488 tubulin (Cytoskeleton, TL488M) was resuspended to 5 mg/mL (45.5 μM) in 4.0 μl of general tubulin buffer (Cytoskeleton, BST01) supplemented with 0.01 volumes of 100 mM GTP (Cytoskeleton, BST06) and 1 μl of microtubules cushion buffer (Cytoskeleton, BST05) to facilitate polymerization.

Techniques: Injection, Staining, Quantitation Assay