general tubulin buffer Search Results


tubulin  (Cytoskeleton Inc)
95
Cytoskeleton Inc tubulin
(A) Confocal images of cells transfected with indicated GFP-tagged TAOK2 constructs immunostained for <t>α-tubulin.</t> Scale bar: 3μm. Magnified images highlight overlap of GFP-tagged TAOK2 <t>with</t> <t>MTs.</t> Scale bar: 5 μm. (B) GFP-tagged deletion constructs, coiled-coil (CC), transmembrane (TMD), amphipathic helix (AH) domains are marked. Domain necessary and sufficient for MT localization is indicated by red box. (C) Coomassie-stained SDS-page gel shows co-sedimentation of indicated proteins with polymerized tubulin. MT binding assay (n = 3) performed with 5 μg of GST or GST-TAOK2-C (1,187–1,235) protein in presence of Taxol stabilized MT. (D) Percentage of protein bound to MT plotted for indicated protein. Error bars: SEM; n = 3; p < 0.0001, one-way ANOVA. (E) Coomassie-stained SDS-page gel shows amount of purified GST-TAOK2-(1,187–1,235) unbound (S) or co-sedimented with polymerized MT (P). (F) Dissociation constant K D derived by the Michaelis-Menten equation. Error bars: SEM; n = 3 replicates. See also .
Tubulin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tubulin/product/Cytoskeleton Inc
Average 95 stars, based on 1 article reviews
tubulin - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
bovine brain tubulin  (Cytoskeleton Inc)
95
Cytoskeleton Inc bovine brain tubulin
(A) Confocal images of cells transfected with indicated GFP-tagged TAOK2 constructs immunostained for <t>α-tubulin.</t> Scale bar: 3μm. Magnified images highlight overlap of GFP-tagged TAOK2 <t>with</t> <t>MTs.</t> Scale bar: 5 μm. (B) GFP-tagged deletion constructs, coiled-coil (CC), transmembrane (TMD), amphipathic helix (AH) domains are marked. Domain necessary and sufficient for MT localization is indicated by red box. (C) Coomassie-stained SDS-page gel shows co-sedimentation of indicated proteins with polymerized tubulin. MT binding assay (n = 3) performed with 5 μg of GST or GST-TAOK2-C (1,187–1,235) protein in presence of Taxol stabilized MT. (D) Percentage of protein bound to MT plotted for indicated protein. Error bars: SEM; n = 3; p < 0.0001, one-way ANOVA. (E) Coomassie-stained SDS-page gel shows amount of purified GST-TAOK2-(1,187–1,235) unbound (S) or co-sedimented with polymerized MT (P). (F) Dissociation constant K D derived by the Michaelis-Menten equation. Error bars: SEM; n = 3 replicates. See also .
Bovine Brain Tubulin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bovine brain tubulin/product/Cytoskeleton Inc
Average 95 stars, based on 1 article reviews
bovine brain tubulin - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier

Image Search Results


(A) Confocal images of cells transfected with indicated GFP-tagged TAOK2 constructs immunostained for α-tubulin. Scale bar: 3μm. Magnified images highlight overlap of GFP-tagged TAOK2 with MTs. Scale bar: 5 μm. (B) GFP-tagged deletion constructs, coiled-coil (CC), transmembrane (TMD), amphipathic helix (AH) domains are marked. Domain necessary and sufficient for MT localization is indicated by red box. (C) Coomassie-stained SDS-page gel shows co-sedimentation of indicated proteins with polymerized tubulin. MT binding assay (n = 3) performed with 5 μg of GST or GST-TAOK2-C (1,187–1,235) protein in presence of Taxol stabilized MT. (D) Percentage of protein bound to MT plotted for indicated protein. Error bars: SEM; n = 3; p < 0.0001, one-way ANOVA. (E) Coomassie-stained SDS-page gel shows amount of purified GST-TAOK2-(1,187–1,235) unbound (S) or co-sedimented with polymerized MT (P). (F) Dissociation constant K D derived by the Michaelis-Menten equation. Error bars: SEM; n = 3 replicates. See also .

Journal: Developmental cell

Article Title: TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules

doi: 10.1016/j.devcel.2021.11.015

Figure Lengend Snippet: (A) Confocal images of cells transfected with indicated GFP-tagged TAOK2 constructs immunostained for α-tubulin. Scale bar: 3μm. Magnified images highlight overlap of GFP-tagged TAOK2 with MTs. Scale bar: 5 μm. (B) GFP-tagged deletion constructs, coiled-coil (CC), transmembrane (TMD), amphipathic helix (AH) domains are marked. Domain necessary and sufficient for MT localization is indicated by red box. (C) Coomassie-stained SDS-page gel shows co-sedimentation of indicated proteins with polymerized tubulin. MT binding assay (n = 3) performed with 5 μg of GST or GST-TAOK2-C (1,187–1,235) protein in presence of Taxol stabilized MT. (D) Percentage of protein bound to MT plotted for indicated protein. Error bars: SEM; n = 3; p < 0.0001, one-way ANOVA. (E) Coomassie-stained SDS-page gel shows amount of purified GST-TAOK2-(1,187–1,235) unbound (S) or co-sedimented with polymerized MT (P). (F) Dissociation constant K D derived by the Michaelis-Menten equation. Error bars: SEM; n = 3 replicates. See also .

Article Snippet: MTs were prepared by polymerizing porcine tubulin (Cytoskeleton inc.) in general tubulin buffer (80mM PIPES pH 6.9, 2mM MgCl 2 , 0.4mM EGTA, Roche Protease Inhibitors) in the presence of 1mM GTP for 20 min at 35C and then diluted further.

Techniques: Transfection, Construct, Staining, SDS Page, Sedimentation, Binding Assay, Purification, Derivative Assay

KEY RESOURCES TABLE

Journal: Developmental cell

Article Title: TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules

doi: 10.1016/j.devcel.2021.11.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MTs were prepared by polymerizing porcine tubulin (Cytoskeleton inc.) in general tubulin buffer (80mM PIPES pH 6.9, 2mM MgCl 2 , 0.4mM EGTA, Roche Protease Inhibitors) in the presence of 1mM GTP for 20 min at 35C and then diluted further.

Techniques: Recombinant, Purification, Software